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Somatic embryogenesis thesis

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Immediate download. Please login to continue. This thesis reports a series of experiments carried out to investigate the process of somatic embryogenesis in conifers, with emphasis on the spruces Picea abies L. Karst and P. Successful regeneration of Sitka spruce plants from somatic embryos is reported.
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Somatic Embryogenesis in Banana (Musa spp.)

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Somatic Embryogenesis | Theories Behind Plant Tissue Culture - passel

Somatic embryogenesis is one of the best examples of the remarkable developmental plasticity of plants, in which committed somatic cells can dedifferentiate and acquire the ability to form an embryo and regenerate an entire plant. In Arabidopsis thaliana, the shoot apices of young seedlings have been reported as an alternative tissue source for somatic embryos SEs besides the widely studied zygotic embryos taken from siliques. Although SE induction from shoots demonstrates the plasticity of plants more clearly than the embryo-to-embryo induction system, the underlying developmental and molecular mechanisms involved are unknown. Here we characterized SE formation from shoot apex explants by establishing a system for time-lapse observation of explants during SE induction. We also established a method to distinguish SE-forming and non-SE-forming explants prior to anatomical SE formation, enabling us to identify distinct transcriptome profiles of these two explants at SE initiation. We show that embryonic fate commitment takes place at day 3 of SE induction and the SE arises directly, not through callus formation, from the base of leaf primordia just beside the shoot apical meristem SAM , where auxin accumulates and shoot-root polarity is formed. The expression domain of a couple of key developmental genes for the SAM transiently expands at this stage.
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- Somatic Embryogenesis

While a large focus of the effort was in instrumentation associated with gravity-driven liquid flows, the work also included extensive trouble-shooting associated with various difficulties of implementation as well as the techniques of maintaining a wide variety of tissue cultures. A temporary immersion bioreactor is tissue culture system in which the liquid nutrient medium is periodically flooded into the culture vessel to provide dissolved nutrients. This is particularly useful for culture plant tissues because constant submergence can lead to being water-logged and difficulties in providing oxygen from the gas phase.
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In this article we will discuss about: 1. Process of Somatic Embryogenesis 2. Embryo Maturation and Synchronisation 3.
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